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Welcome to the Human Pain Genetics Lab!


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Luda Diatchenko, MD, Ph.D

Canada Excellence Research Chair in Human Pain Genetics

The Human Pain Genetics lab investigates the psychological, molecular, cellular, and genetic pathways that mediate both acute and persistent pain states. The primary goal of these efforts is to identify critical elements of human genetic variability contributing to pain sensitivity and pathophysiological pain states that will enable individualized treatments and therapies. Other related research endeavors include identifying the molecular hierarchy of functional single-nucleotide polymorphisms (SNPs) and SNP-dependent regulation of gene expression underlying molecular pain signalling. Answering these questions requires collaboration with experts in both clinical and basic biological sciences. Such collaborative activities allow the Human Pain Genetics lab to take basic genetic findings all the way from human association studies, to molecular and cellular mechanisms, to animal models, and ultimately to human clinical trials.

Pain perception is one of the most complicated measurable traits, because it is composed of an aggregate of several other measurable phenotypes associated with peripheral and central nervous system dynamics, stress responsiveness, and inflammatory state. It is generally accepted that complex traits like pain perception result from the interplay between environmental exposures and multiple genetic variants; however, little is known about the nature of these genetic variants. Because of the established roles of environmental exposures and the commonly held view that pain perception is an unquantifiable "subjective" experience, a genetic basis for pain perception has long been questioned. Recent and rapidly developing discoveries in the field of pain genetics have provided evidence for a substantial role for genetic background on pain perception and clinical pain phenotypes. These findings provide unique opportunities to identify new genetic variants that contribute to pain phenotypes.

Click for a recent talk on pain aimed at a general audience: [podcast] [YouTube video].

Acute and Persistent Pain States
Translational Clock of COMT Gene

Organization Logos
Our Team

People


The Diatchenko Lab comprises a diverse group of scientists, not only with a wide range of areas of expertise such as molecular biology, neuroscience, dentistry, genetics, medicine, and bioinformatics, but also with different levels of training, from undergraduate students completing their honors projects to postdoctoral fellows and academic faculty. Cultural diversity is also embraced as one of our great assets, with members joining us from many parts of the world, creating a stimulating and exciting environment for professional growth. Former trainees from our lab have also gone on to develop successful careers in a variety of fields, both in academia and the private sector.

Name Position More Information
Rodrigo Benavides, MD Post-doctorate

Phone: 514-398-2717
E-mail: rodrigo.benevides@mail.mcgill.ca
Bio

Photograph of Rodrigo Benavides, MD
Stefano Cattaneo, MD, BSc MSc Trainee

Phone: 514-398-2717
E-mail: stefano.cattaneo@mail.mcgill.ca
CV

Photograph of Stefano Cattaneo, MD, BSc
Anne-Julie Chabot-Doré, PhD Post-doctorate

Phone: 514-398-2717
E-mail: anne-julie.chabot-dore@mail.mcgill.ca

Photograph of Anne-Julie Chabot-Doré, PhD
Luda Diatchenko, MD, PhD Principal Investigator

Phone: 514-398-2878
E-mail: luda.diatchenko@mcgill.ca
CV Bio

Photograph of Luda Diatchenko, MD, PhD
Pavel Gris, MD, PhD Senior Research Associate
Lab Manager

Phone: 514-398-3176
E-mail: pavelgris@gmail.com

Photograph of Pavel Gris, MD, PhD
Samar Khoury, PhD Post-doctorate

Phone: 514-398-2833
E-mail: samar.khoury2@mail.mcgill.ca
CV

Photograph of Samar Khoury, PhD
Richard Klares Undergraduate

Phone: 514-398-2717
E-mail: richard.klares@mail.mcgill.ca

Photograph of Richard Klares
Ryan Lichtenwalter, PhD Data Analyst

Phone: 514-398-3176
E-mail: ryan.lichtenwalter@mcgill.ca
CV Bio

Photograph of Ryan Lichtenwalter, PhD
Sarah Jane Martinez, MSc Chief Research Technician

Phone: 514-398-5971
E-mail: sarah.martinez@mcgill.ca
Bio

Photograph of Sarah Jane Martinez, MSc
Carol Meloto, DDS, PhD Post-doctorate

Phone: 514-398-2833
E-mail: carol.meloto@mail.mcgill.ca
CV

Photograph of Carol Meloto, DDS, PhD
Marc Parisien, PhD Research Associate

Phone: 514-398-2717
E-mail: marc.parisien@mcgill.ca
CV Bio

Photograph of Marc Parisien, PhD
Marjo Piltonen, PhD Post-doctorate

Phone: 514-398-2833
E-mail: marjo.piltonen@mail.mcgill.ca

Photograph of Marjo Piltonen, PhD
Alexander Samoshkin, PhD Faculty Lecturer

Phone: 514-398-6592
E-mail: alex.samoshkin@mcgill.ca

Photograph of Alexander Samoshkin, PhD
Julia Segal Undergraduate

Phone: 514-398-2717
E-mail: julia.segal@mail.mcgill.ca

Photograph of Julia Segal
Nicol Tugarinov Undergraduate

Phone: 514-398-2717
E-mail: nicol.tugarinov@mail.mcgill.ca
Bio

Photograph of Nicol Tugarinov
Vivek Verma, BDS, MSc PhD Candidate

Phone: 514-398-2717
E-mail: vivek.verma@mail.mcgill.ca
CV

Photograph of Vivek Verma, BDS, MSc
Maria Verner Undergraduate

Phone: 514-398-2717
E-mail: maria.verner@mail.mcgill.ca

Photograph of Maria Verner
Natalie Zacchia, MPH Clinical Research Administrator

Phone: 514-398-8366
E-mail: nat.zacchia@mcgill.ca
CV

Photograph of Natalie Zacchia, MPH
Katerina Zorina-Lichtenwalter, MA PhD Candidate

Phone: 514-398-2833
E-mail: katerina.lichtenwalter@mail.mcgill.ca
CV

Photograph of Katerina Zorina-Lichtenwalter, MA
Name Position More Information
Diversity Map

Past Members

Name Position More Information
Xia (Shawn) Wen Undergraduate
Photograph of Xia (Shawn) Wen
Meijuan Niu Lab Manager
Photograph of Meijuan Niu
Hao Huang Lab Manager
Photograph of Hao Huang
Yerkebulan Talzhanov Post-doctorate
Photograph of Yerkebulan Talzhanov
Lisanne Plein Master's Candidate
Photograph of Lisanne Plein
Name Position More Information
Publications

Publications


Peer-Reviewed Journal Articles
Agonist-dependence of functional properties for common nonsynonymous variants of human transient receptor potential vanilloid 1. Wang, Joseph, Diatchenko, Ro, Chung. Pain, 157(7). 2016.
Painful Temporomandibular Disorder: Decade of Discovery from OPPERA Studies. Slade, Ohrbach, Greenspan, Fillingim, Bair, Sanders, Dubner, Diatchenko, Meloto, Smith, Maixner. Journal of dental research. 2016.
Identification of clusters of individuals relevant to temporomandibular disorders and other chronic pain conditions: the OPPERA study. Bair, Gaynor, Slade, Ohrbach, Fillingim, Greenspan, Dubner, Smith, Diatchenko, Maixner. Pain, 157(6). 2016.
Human Genetic Variability Contributes to Postoperative Morphine Consumption. De Gregori, Diatchenko, Ingelmo, Napolioni, Klepstad, Belfer, Molinaro, Garbin, Ranzani, Alberio, Normanno, Lovisari, Somaini, Govoni, Mura, Bugada, Niebel, Zorzetto, De Gregori, Molinaro, Fanelli, Allegri. The journal of pain : official journal of the American Pain Society, 17(5). 2016.
Genetic predictors of human chronic pain conditions. Zorina-Lichtenwalter, Meloto, Khoury, Diatchenko. Neuroscience. 2016.
Modification of COMT-dependent pain sensitivity by psychological stress and sex. Meloto, Bortsov, Bair, Helgeson, Ostrom, Smith, Dubner, Slade, Fillingim, Greenspan, Ohrbach, Maixner, McLean, Diatchenko. Pain, 157(4). 2016.
N(6)-Methyladenosine Modification in a Long Noncoding RNA Hairpin Predisposes Its Conformation to Protein Binding. Zhou, Parisien, Dai, Liu, Diatchenko, Sachleben, Pan. Journal of molecular biology, 428(5 Pt A). 2016.
Dual allosteric modulation of opioid antinociceptive potency by α2A-adrenoceptors. Chabot-Doré, Millecamps, Naso, Devost, Trieu, Piltonen, Diatchenko, Fairbanks, Wilcox, Hébert, Stone. Neuropharmacology, 99. 2015.
Differences in the Antinociceptive Effects and Binding Properties of Propranolol and Bupranolol Enantiomers. Martin, Piltonen, Gauthier, Convertino, Acland, Dokholyan, Mogil, Diatchenko, Maixner. The journal of pain : official journal of the American Pain Society, 16(12). 2015.
Genome-wide association meta-analyses to identify common genetic variants associated with hallux valgus in Caucasian and African Americans. Hsu, Liu, Hannan, Maixner, Smith, Diatchenko, Golightly, Menz, Kraus, Doherty, Wilson, Jordan. Journal of medical genetics, 52(11). 2015.
Neuropathic pain phenotyping by international consensus (NeuroPPIC) for genetic studies: a NeuPSIG systematic review, Delphi survey, and expert panel recommendations. van Hecke, Kamerman, Attal, Baron, Bjornsdottir, Bennett, Bennett, Bouhassira, Diatchenko, Freeman, Freynhagen, Haanpää, Jensen, Raja, Rice, Seltzer, Thorgeirsson, Yarnitsky, Smith. Pain, 156(11). 2015.
μ-Opioid receptor 6-transmembrane isoform: A potential therapeutic target for new effective opioids. Convertino, Samoshkin, Gauthier, Gold, Maixner, Dokholyan, Diatchenko. Progress in neuro-psychopharmacology & biological psychiatry, 62. 2015.
Molecular genetic mechanisms of allelic specific regulation of murine Comt expression. Segall, Shabalina, Meloto, Wen, Cunningham, Tarantino, Wiltshire, Gauthier, Tohyama, Martin, Mogil, Diatchenko. Pain, 156(10). 2015.
COMT gene locus: new functional variants. Meloto, Segall, Smith, Parisien, Shabalina, Rizzatti-Barbosa, Gauthier, Tsao, Convertino, Piltonen, Slade, Fillingim, Greenspan, Ohrbach, Knott, Maixner, Zaykin, Dokholyan, Reenilä, Männistö, Diatchenko. Pain, 156(10). 2015.
COMT Diplotype Amplifies Effect of Stress on Risk of Temporomandibular Pain. Slade, Sanders, Ohrbach, Bair, Maixner, Greenspan, Fillingim, Smith, Diatchenko. Journal of dental research, 94(9). 2015.
OPRM1 receptor as new biomarker to help the prediction of post mastectomy pain and recurrence in breast cancer. De Gregori, Diatchenko, Belfer, Allegri. Minerva anestesiologica, 81(8). 2015.
The nicotinic α6 subunit gene determines variability in chronic pain sensitivity via cross-inhibition of P2X2/3 receptors. Wieskopf, Mathur, Limapichat, Post, Al-Qazzaz, Sorge, Martin, Zaykin, Smith, Freitas, Austin, Dai, Zhang, Marcovitz, Tuttle, Slepian, Clarke, Drenan, Janes, Al Sharari, Segall, Aasvang, Lai, Bittner, Richards, Slade, Kehlet, Walker, Maskos, Changeux, Devor, Maixner, Diatchenko, Belfer, Dougherty, Su, Lummis, Imad Damaj, Lester, Patapoutian, Mogil. Science translational medicine, 7(287). 2015.
Subgrouping of low back pain patients for targeting treatments: evidence from genetic, psychological, and activity-related behavioral approaches. Huijnen, Rusu, Scholich, Meloto, Diatchenko. The Clinical journal of pain, 31(2). 2015.
Carbonic anhydrase-8 regulates inflammatory pain by inhibiting the ITPR1-cytosolic free calcium pathway. Zhuang, Keeler, Grant, Bianchi, Fu, Zhang, Erasso, Cui, Wiltshire, Li, Hao, Sarantopoulos, Candiotti, Wishnek, Smith, Maixner, Diatchenko, Martin, Levitt. PloS one, 10(3). 2015.
Differential Regulation of 6- and 7-Transmembrane Helix Variants of μ-Opioid Receptor in Response to Morphine Stimulation. Convertino, Samoshkin, Viet, Gauthier, Li Fraine, Sharif-Naeini, Schmidt, Maixner, Diatchenko, Dokholyan. PloS one, 10(11). 2015.
Quantitative H2S-mediated protein sulfhydration reveals metabolic reprogramming during the integrated stress response. Gao, Krokowski, Guan, Bederman, Majumder, Parisien, Diatchenko, Kabil, Willard, Banerjee, Wang, Bebek, Evans, Fox, Gerson, Hoppel, Liu, Arvan, Hatzoglou. eLife, 4. 2015.
Structural and functional interactions between six-transmembrane μ-opioid receptors and β2-adrenoreceptors modulate opioid signaling. Samoshkin, Convertino, Viet, Wieskopf, Kambur, Marcovitz, Patel, Stone, Kalso, Mogil, Schmidt, Maixner, Dokholyan, Diatchenko. Scientific reports, 5. 2015.
Epistasis between polymorphisms in COMT, ESR1, and GCH1 influences COMT enzyme activity and pain. Smith, Reenilä, Männistö, Slade, Maixner, Diatchenko, Nackley. Pain, 155(11). 2014.
Letting the gene out of the bottle: OPRM1 interactions. Belfer, Young, Diatchenko. Anesthesiology, 121(4). 2014.
Complex multilocus effects of catechol-O-methyltransferase haplotypes predict pain and pain interference 6 weeks after motor vehicle collision. Bortsov, Diatchenko, McLean. Neuromolecular medicine, 16(1). 2014.
Differential expression of the alternatively spliced OPRM1 isoform μ-opioid receptor-1K in HIV-infected individuals. Dever, Costin, Xu, El-Hage, Balinang, Samoshkin, O'Brien, McRae, Diatchenko, Knapp, Hauser. AIDS (London, England), 28(1). 2014.
Multivariable modeling of phenotypic risk factors for first-onset TMD: the OPPERA prospective cohort study. Bair, Ohrbach, Fillingim, Greenspan, Dubner, Diatchenko, Helgeson, Knott, Maixner, Slade. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Summary of findings from the OPPERA prospective cohort study of incidence of first-onset temporomandibular disorder: implications and future directions. Slade, Fillingim, Sanders, Bair, Greenspan, Ohrbach, Dubner, Diatchenko, Smith, Knott, Maixner. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Study protocol, sample characteristics, and loss to follow-up: the OPPERA prospective cohort study. Bair, Brownstein, Ohrbach, Greenspan, Dubner, Fillingim, Maixner, Smith, Diatchenko, Gonzalez, Gordon, Lim, Ribeiro-Dasilva, Dampier, Knott, Slade. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Signs and symptoms of first-onset TMD and sociodemographic predictors of its development: the OPPERA prospective cohort study. Slade, Bair, Greenspan, Dubner, Fillingim, Diatchenko, Maixner, Knott, Ohrbach. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Clinical orofacial characteristics associated with risk of first-onset TMD: the OPPERA prospective cohort study. Ohrbach, Bair, Fillingim, Gonzalez, Gordon, Lim, Ribeiro-Dasilva, Diatchenko, Dubner, Greenspan, Knott, Maixner, Smith, Slade. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Pain sensitivity and autonomic factors associated with development of TMD: the OPPERA prospective cohort study. Greenspan, Slade, Bair, Dubner, Fillingim, Ohrbach, Knott, Diatchenko, Liu, Maixner. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Psychological factors associated with development of TMD: the OPPERA prospective cohort study. Fillingim, Ohrbach, Greenspan, Knott, Diatchenko, Dubner, Bair, Baraian, Mack, Slade, Maixner. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Genetic variants associated with development of TMD and its intermediate phenotypes: the genetic architecture of TMD in the OPPERA prospective cohort study. Smith, Mir, Bair, Slade, Dubner, Fillingim, Greenspan, Ohrbach, Knott, Weir, Maixner, Diatchenko. The journal of pain : official journal of the American Pain Society, 14(12 Suppl). 2013.
Facial pain with localized and widespread manifestations: separate pathways of vulnerability. Slade, Smith, Zaykin, Tchivileva, Gibson, Yuryev, Mazo, Bair, Fillingim, Ohrbach, Greenspan, Maixner, Diatchenko. Pain, 154(11). 2013.
Multisystem dysregulation in painful temporomandibular disorders. Chen, Nackley, Miller, Diatchenko, Maixner. The journal of pain : official journal of the American Pain Society, 14(9). 2013.
Pain modality- and sex-specific effects of COMT genetic functional variants. Belfer, Segall, Lariviere, Smith, Dai, Slade, Rashid, Mogil, Campbell, Edwards, Liu, Bair, Maixner, Diatchenko. Pain, 154(8). 2013.
Polymorphisms in the glucocorticoid receptor co-chaperone FKBP5 predict persistent musculoskeletal pain after traumatic stress exposure. Bortsov, Smith, Diatchenko, Soward, Ulirsch, Rossi, Swor, Hauda, Peak, Jones, Holbrook, Rathlev, Foley, Lee, Collette, Domeier, Hendry, McLean. Pain, 154(8). 2013.
Sleep apnea symptoms and risk of temporomandibular disorder: OPPERA cohort. Sanders, Essick, Fillingim, Knott, Ohrbach, Greenspan, Diatchenko, Maixner, Dubner, Bair, Miller, Slade. Journal of dental research, 92(7 Suppl). 2013.
The phenotypic and genetic signatures of common musculoskeletal pain conditions. Diatchenko, Fillingim, Smith, Maixner. Nature reviews. Rheumatology, 9(6). 2013.
Correlation of transcription of MALAT-1, a novel noncoding RNA, with deregulated expression of tumor suppressor p53 in small DNA tumor virus models. Jeffers, Duan, Ellies, Seaman, Burger-Calderon, Diatchenko, Webster-Cyriaque. Journal of cancer therapy, 4(3). 2013.
Relationship between temporomandibular disorders, widespread palpation tenderness, and multiple pain conditions: a case-control study. Chen, Slade, Lim, Miller, Maixner, Diatchenko. The journal of pain : official journal of the American Pain Society, 13(10). 2012.
Janus molecule I: dichotomous effects of COMT in neuropathic vs nociceptive pain modalities. Segall, Maixner, Belfer, Wiltshire, Seltzer, Diatchenko. CNS & neurological disorders drug targets, 11(3). 2012.
Genetically determined P2X7 receptor pore formation regulates variability in chronic pain sensitivity. Sorge, Trang, Dorfman, Smith, Beggs, Ritchie, Austin, Zaykin, Vander Meulen, Costigan, Herbert, Yarkoni-Abitbul, Tichauer, Livneh, Gershon, Zheng, Tan, John, Slade, Jordan, Woolf, Peltz, Maixner, Diatchenko, Seltzer, Salter, Mogil. Nature medicine, 18(4). 2012.
Large candidate gene association study reveals genetic risk factors and therapeutic targets for fibromyalgia. Smith, Maixner, Fillingim, Slade, Gracely, Ambrose, Zaykin, Hyde, John, Tan, Maixner, Diatchenko. Arthritis and rheumatism, 64(2). 2012.
Excess risk of temporomandibular disorder associated with cigarette smoking in young adults. Sanders, Maixner, Nackley, Diatchenko, By, Miller, Slade. The journal of pain : official journal of the American Pain Society, 13(1). 2012.
Catechol-O-methyltransferase genotype predicts pain severity in hospitalized burn patients. Orrey, Bortsov, Hoskins, Shupp, Jones, Cicuto, Hwang, Jordan, Holmes, Haith, Roane, Diatchenko, Cairns, McLean. Journal of burn care & research : official publication of the American Burn Association, 33(4). 2012.
Serotonin-induced hypersensitivity via inhibition of catechol O-methyltransferase activity. Tsao, Wieskopf, Rashid, Sorge, Redler, Segall, Mogil, Maixner, Dokholyan, Diatchenko. Molecular pain, 8. 2012.
Construction of a global pain systems network highlights phospholipid signaling as a regulator of heat nociception. Neely, Rao, Costigan, Mair, Racz, Milinkeviciute, Meixner, Nayanala, Griffin, Belfer, Dai, Smith, Diatchenko, Marengo, Haubner, Novatchkova, Gibson, Maixner, Pospisilik, Hirsch, Whishaw, Zimmer, Gupta, Sasaki, Kanaho, Sasaki, Kress, Woolf, Penninger. PLoS genetics, 8(12). 2012.
Cytokine biomarkers and chronic pain: association of genes, transcription, and circulating proteins with temporomandibular disorders and widespread palpation tenderness. Slade, Conrad, Diatchenko, Rashid, Zhong, Smith, Rhodes, Medvedev, Makarov, Maixner, Nackley. Pain, 152(12). 2011.
Relax, you won't feel the pain. Wiltshire, Maixner, Diatchenko. Nature neuroscience, 14(12). 2011.
Elucidation of mu-Opioid Gene Structure: How Genetics Can Help Predict Responses to Opioids. Diatchenko, Robinson, Maixner. European journal of pain supplements, 5(2). 2011.
Structural basis for μ-opioid receptor binding and activation. Serohijos, Yin, Ding, Gauthier, Gibson, Maixner, Dokholyan, Diatchenko. Structure (London, England : 1993), 19(11). 2011.
Summary of findings from the OPPERA baseline case-control study: implications and future directions. Fillingim, Slade, Diatchenko, Dubner, Greenspan, Knott, Ohrbach, Maixner. The journal of pain : official journal of the American Pain Society, 12(11 Suppl). 2011.
Study methods, recruitment, sociodemographic findings, and demographic representativeness in the OPPERA study. Slade, Bair, By, Mulkey, Baraian, Rothwell, Reynolds, Miller, Gonzalez, Gordon, Ribeiro-Dasilva, Lim, Greenspan, Dubner, Fillingim, Diatchenko, Maixner, Dampier, Knott, Ohrbach. The journal of pain : official journal of the American Pain Society, 12(11 Suppl). 2011.
Orofacial pain prospective evaluation and risk assessment study--the OPPERA study. Maixner, Diatchenko, Dubner, Fillingim, Greenspan, Knott, Ohrbach, Weir, Slade. The journal of pain : official journal of the American Pain Society, 12(11 Suppl). 2011.
Potential autonomic risk factors for chronic TMD: descriptive data and empirically identified domains from the OPPERA case-control study. Maixner, Greenspan, Dubner, Bair, Mulkey, Miller, Knott, Slade, Ohrbach, Diatchenko, Fillingim. The journal of pain : official journal of the American Pain Society, 12(11 Suppl). 2011.
Potential genetic risk factors for chronic TMD: genetic associations from the OPPERA case control study. Smith, Maixner, Greenspan, Dubner, Fillingim, Ohrbach, Knott, Slade, Bair, Gibson, Zaykin, Weir, Maixner, Diatchenko. The journal of pain : official journal of the American Pain Society, 12(11 Suppl). 2011.
Disruptive mRNA folding increases translational efficiency of catechol-O-methyltransferase variant. Tsao, Shabalina, Gauthier, Dokholyan, Diatchenko. Nucleic acids research, 39(14). 2011.
Catechol O-methyltransferase haplotype predicts immediate musculoskeletal neck pain and psychological symptoms after motor vehicle collision. McLean, Diatchenko, Lee, Swor, Domeier, Jones, Jones, Reed, Harris, Maixner, Clauw, Liberzon. The journal of pain : official journal of the American Pain Society, 12(1). 2011.
Structural mechanism of S-adenosyl methionine binding to catechol O-methyltransferase. Tsao, Diatchenko, Dokholyan. PloS one, 6(8). 2011.
A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely, Hess, Costigan, Keene, Goulas, Langeslag, Griffin, Belfer, Dai, Smith, Diatchenko, Gupta, Xia, Amann, Kreitz, Heindl-Erdmann, Wolz, Ly, Arora, Sarangi, Dan, Novatchkova, Rosenzweig, Gibson, Truong, Schramek, Zoranovic, Cronin, Angjeli, Brune, Dietzl, Maixner, Meixner, Thomas, Pospisilik, Alenius, Kress, Subramaniam, Garrity, Bellen, Woolf, Penninger. Cell, 143(4). 2010.
Comt1 genotype and expression predicts anxiety and nociceptive sensitivity in inbred strains of mice. Segall, Nackley, Diatchenko, Lariviere, Lu, Marron, Grabowski-Boase, Walker, Slade, Gauthier, Bailey, Steffy, Maynard, Tarantino, Wiltshire. Genes, brain, and behavior, 9(8). 2010.
Multiple chronic pain states are associated with a common amino acid-changing allele in KCNS1. Costigan, Belfer, Griffin, Dai, Barrett, Coppola, Wu, Kiselycznyk, Poddar, Lu, Diatchenko, Smith, Cobos, Zaykin, Allchorne, Gershon, Livneh, Shen, Nikolajsen, Karppinen, Männikkö, Kelempisioti, Goldman, Maixner, Geschwind, Max, Seltzer, Woolf. Brain : a journal of neurology, 133(9). 2010.
Pain perception is altered by a nucleotide polymorphism in SCN9A. Reimann, Cox, Belfer, Diatchenko, Zaykin, McHale, Drenth, Dai, Wheeler, Sanders, Wood, Wu, Karppinen, Nikolajsen, Männikkö, Max, Kiselycznyk, Poddar, Te Morsche, Smith, Gibson, Kelempisioti, Maixner, Gribble, Woods. Proceedings of the National Academy of Sciences of the United States of America, 107(11). 2010.
Assessing potential functionality of catechol-O-methyltransferase (COMT) polymorphisms associated with pain sensitivity and temporomandibular joint disorders. Nackley, Diatchenko. Methods in molecular biology (Clifton, N.J.), 617. 2010.
Molecular assays for characterization of alternatively spliced isoforms of the u opioid receptor (MOR). Gris, Cheng, Pierson, Maixner, Diatchenko. Methods in molecular biology (Clifton, N.J.), 617. 2010.
A novel alternatively spliced isoform of the mu-opioid receptor: functional antagonism. Gris, Gauthier, Cheng, Gibson, Gris, Laur, Pierson, Wentworth, Nackley, Maixner, Diatchenko. Molecular pain, 6. 2010.
Signaling pathways mediating beta3-adrenergic receptor-induced production of interleukin-6 in adipocytes. Tchivileva, Tan, Gambarian, Nackley, Medvedev, Romanov, Flood, Maixner, Makarov, Diatchenko. Molecular immunology, 46(11-12). 2009.
Expansion of the human mu-opioid receptor gene architecture: novel functional variants. Shabalina, Zaykin, Gris, Ogurtsov, Gauthier, Shibata, Tchivileva, Belfer, Mishra, Kiselycznyk, Wallace, Staud, Spiridonov, Max, Goldman, Fillingim, Maixner, Diatchenko. Human molecular genetics, 18(6). 2009.
Haplotype associations with quantitative traits in the presence of complex multilocus and heterogeneous effects. Shibata, Diatchenko, Zaykin. Genetic epidemiology, 33(1). 2009.
Characterization of NF-kB-mediated inhibition of catechol-O-methyltransferase. Tchivileva, Nackley, Qian, Wentworth, Conrad, Diatchenko. Molecular pain, 5. 2009.
Low enzymatic activity haplotypes of the human catechol-O-methyltransferase gene: enrichment for marker SNPs. Nackley, Shabalina, Lambert, Conrad, Gibson, Spiridonov, Satterfield, Diatchenko. PloS one, 4(4). 2009.
Orthodontic Treatment, Genetic Factors and Risk of Temporomandibular Disorder. Slade, Diatchenko, Ohrbach, Maixner. Seminars in orthodontics, 14(2). 2008.
Homogeneous reporter system enables quantitative functional assessment of multiple transcription factors. Romanov, Medvedev, Gambarian, Poltoratskaya, Moeser, Medvedeva, Gambarian, Diatchenko, Makarov. Nature methods, 5(3). 2008.
Genetic architecture of human pain perception. Diatchenko, Nackley, Tchivileva, Shabalina, Maixner. Trends in genetics : TIG, 23(12). 2007.
Influence of psychological factors on risk of temporomandibular disorders. Slade, Diatchenko, Bhalang, Sigurdsson, Fillingim, Belfer, Max, Goldman, Maixner. Journal of dental research, 86(11). 2007.
Responses to Drs. Kim and Dionne regarding comments on Diatchenko, et al. Catechol-O-methyltransferase gene polymorphisms are associated with multiple pain-evoking stimuli. Pain 2006; 125: 216-24. Diatchenko, Slade, Nackley, Maixner. Pain, 129(3). 2007.
Catechol-O-methyltransferase inhibition increases pain sensitivity through activation of both beta2- and beta3-adrenergic receptors. Nackley, Tan, Fecho, Flood, Diatchenko, Maixner. Pain, 128(3). 2007.
Beta2 adrenergic receptor activation stimulates pro-inflammatory cytokine production in macrophages via PKA- and NF-kappaB-independent mechanisms. Tan, Nackley, Satterfield, Maixner, Diatchenko, Flood. Cellular signalling, 19(2). 2007.
Human catechol-O-methyltransferase haplotypes modulate protein expression by altering mRNA secondary structure. Nackley, Shabalina, Tchivileva, Satterfield, Korchynskyi, Makarov, Maixner, Diatchenko. Science (New York, N.Y.), 314(5807). 2006.
Catechol-O-methyltransferase gene polymorphisms are associated with multiple pain-evoking stimuli. Diatchenko, Nackley, Slade, Bhalang, Belfer, Max, Goldman, Maixner. Pain, 125(3). 2006.
GTP cyclohydrolase and tetrahydrobiopterin regulate pain sensitivity and persistence. Tegeder, Costigan, Griffin, Abele, Belfer, Schmidt, Ehnert, Nejim, Marian, Scholz, Wu, Allchorne, Diatchenko, Binshtok, Goldman, Adolph, Sama, Atlas, Carlezon, Parsegian, Lötsch, Fillingim, Maixner, Geisslinger, Max, Woolf. Nature medicine, 12(11). 2006.
Idiopathic pain disorders--pathways of vulnerability. Diatchenko, Nackley, Slade, Fillingim, Maixner. Pain, 123(3). 2006.
Three major haplotypes of the beta2 adrenergic receptor define psychological profile, blood pressure, and the risk for development of a common musculoskeletal pain disorder. Diatchenko, Anderson, Slade, Fillingim, Shabalina, Higgins, Sama, Belfer, Goldman, Max, Weir, Maixner. American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, 141B(5). 2006.
Perspectives on the genetic basis of opioid-induced hyperalgesia. Nackley, Diatchenko, Maixner. Anesthesiology, 104(5). 2006.
Identification of novel mediators of NF-kappaB through genome-wide survey of monocyte adherence-induced genes. Diatchenko, Romanov, Malinina, Clarke, Tchivilev, Li, Makarov. Journal of leukocyte biology, 78(6). 2005.
Genetic basis for individual variations in pain perception and the development of a chronic pain condition. Diatchenko, Slade, Nackley, Bhalang, Sigurdsson, Belfer, Goldman, Xu, Shabalina, Shagin, Max, Makarov, Maixner. Human molecular genetics, 14(1). 2005.
Gene expression analysis of purified hematopoietic stem cells and committed progenitors. Terskikh, Miyamoto, Chang, Diatchenko, Weissman. Blood, 102(1). 2003.
Generation of full-length cDNA libraries enriched for differentially expressed genes. Zhumabayeva, Chang, McKinley, Diatchenko, Siebert. Methods in molecular biology (Clifton, N.J.), 221. 2003.
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer, Rubin, Hong, Stapleton, Soares, Bonaldo, Casavant, Scheetz, Brownstein, Usdin, Toshiyuki, Carninci, Prange, Raha, Loquellano, Peters, Abramson, Mullahy, Bosak, McEwan, McKernan, Malek, Gunaratne, Richards, Worley, Hale, Garcia, Gay, Hulyk, Villalon, Muzny, Sodergren, Lu, Gibbs, Fahey, Helton, Ketteman, Madan, Rodrigues, Sanchez, Whiting, Madan, Young, Shevchenko, Bouffard, Blakesley, Touchman, Green, Dickson, Rodriguez, Grimwood, Schmutz, Myers, Butterfield, Krzywinski, Skalska, Smailus, Schnerch, Schein, Jones, Marra. Proceedings of the National Academy of Sciences of the United States of America, 99(26). 2002.
Subtractive cloning: new genes for studying inflammatory disorders. Rebrikov, Desai, Kogan, Thornton, Diatchenko. Annals of periodontology / the American Academy of Periodontology, 7(1). 2002.
Gene expression profiling in RAS oncogene-transformed cell lines and in solid tumors using subtractive suppression hybridization and cDNA arrays. Sers, Tchernitsa, Zuber, Diatchenko, Zhumabayeva, Desai, Htun, Hyder, Wiechen, Agoulnik, Scharff, Siebert, Schäfer. Advances in enzyme regulation, 42. 2002.
Caveolin-1 is down-regulated in human ovarian carcinoma and acts as a candidate tumor suppressor gene. Wiechen, Diatchenko, Agoulnik, Scharff, Schober, Arlt, Zhumabayeva, Siebert, Dietel, Schäfer, Sers. The American journal of pathology, 159(5). 2001.
Generation of full-length cDNA libraries enriched for differentially expressed genes for functional genomics. Zhumabayeva, Chang, McKinley, Diatchenko, Siebert. BioTechniques, 30(3). 2001.
Use of SMART-generated cDNA for gene expression studies in multiple human tumors. Zhumabayeva, Diatchenko, Chenchik, Siebert. BioTechniques, 30(1). 2001.
Structure and regulation of the mouse ing1 gene. Three alternative transcripts encode two phd finger proteins that have opposite effects on p53 function. Zeremski, Hill, Kwek, Grigorian, Gurova, Garkavtsev, Diatchenko, Koonin, Gudkov. The Journal of biological chemistry, 274(45). 1999.
Amplification of cDNA ends based on template-switching effect and step-out PCR. Matz, Shagin, Bogdanova, Britanova, Lukyanov, Diatchenko, Chenchik. Nucleic acids research, 27(6). 1999.
Suppression subtractive hybridization: a versatile method for identifying differentially expressed genes. Diatchenko, Lukyanov, Lau, Siebert. Methods in enzymology, 303. 1999.
PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori. Akopyants, Fradkov, Diatchenko, Hill, Siebert, Lukyanov, Sverdlov, Berg. Proceedings of the National Academy of Sciences of the United States of America, 95(22). 1998.
Stress-induced secretion of growth inhibitors: a novel tumor suppressor function of p53. Komarova, Diatchenko, Rokhlin, Hill, Wang, Krivokrysenko, Feinstein, Gudkov. Oncogene, 17(9). 1998.
Sequence-independent method for in vitro generation of nested deletions for sequencing large DNA fragments. Fradkov, Lukyanov, Matz, Diatchenko, Siebert, Lukyanov. Analytical biochemistry, 258(1). 1998.
Differential screening of a subtracted cDNA library: a method to search for genes preferentially expressed in multiple tissues. Jin, Cheng, Diatchenko, Siebert, Huang. BioTechniques, 23(6). 1997.
Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect. Lukyanov, Diatchenko, Chenchik, Nanisetti, Siebert, Usman, Matz, Lukyanov. Biochemical and biophysical research communications, 230(2). 1997.
Equalizing cDNA subtraction based on selective suppression of polymerase chain reaction: cloning of Jurkat cell transcripts induced by phytohemaglutinin and phorbol 12-myristate 13-acetate. Gurskaya, Diatchenko, Chenchik, Siebert, Khaspekov, Lukyanov, Vagner, Ermolaeva, Lukyanov, Sverdlov. Analytical biochemistry, 240(1). 1996.
Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Diatchenko, Lau, Campbell, Chenchik, Moqadam, Huang, Lukyanov, Lukyanov, Gurskaya, Sverdlov, Siebert. Proceedings of the National Academy of Sciences of the United States of America, 93(12). 1996.
Peer-Reviewed Journal Articles
Books and Book Chapters
An Introduction to Pain. Mogil, Diatchenko, Fillingim. In: Pain Genetics: Refresher Course Chapter. IASP 2014 Meeting, Buenos Aires, Argentina. 2014.
Translating Genetic Knowledge into Clinical Practice for Musculoskeletal Pain Conditions. Kuo, Diatchenko, Zaykin. In: Genetics of Human Pain Perception: Basic to Translational Science. Editors: Belfer, Diatchenko. Oxford, UK. 2014. Chapter 7: pp. 99-103.
Discovering Multilocus Associations with Complex Pain Phenotype. Diatchenko, Smith, Maixner. In: Genetics of Human Pain Perception: Basic to Translational Science. Editors: Belfer, Diatchenko. Oxford, UK. 2014. Chapter 10: pp. 147-156.
COMT is a Major Contributor to Pain Perception: from Humans to Rats and Back to Humans. Diatchenko. In: Catecholamine Research in the 21st Century: Abstracts and Graphical Abstracts, 10th International Catecholamine Symposium 2012. Editors: Eiden. 2013.
The search for human pain genes using whole genome approaches: achievements, failures and promises. Smith, Tchivileva, Maixner, Diatchenko. In: Pain 2010 - An Updated Review: Refresher Course Syllabus. Editors: Mogil. Seattle: IASP Press . 2012.
The search for human pain genes using whole genome approaches: achievements, failures and promises. Smith, Tchivileva, Maixner, Diatchenko. In: Pain Genes for Unraveling Pain: A Course for Non-Geneticists. Editors: Mogil. Seattle: IASP Press. 2010. pp. 255-265.
Generation of full-length cDNA libraries enriched for differentially expressed genes. Zhumabayeva, Chang, McKinley, Diatchenko, Siebert. In: Methods in Molecular Biology. Editors: Walker. Totowa: Humana Press. 2003. pp. 221,223-37.
SSH subtractive cloning: comprehensive survey of genome differences in prokaryotes. Rebrikov, Kogan, Diatchenko. In: Applications of Genomics and Proteomics for Analysis of Bacterial Warfare Agents. Editors: DelVecchio, Krcmery. Amsterdam: IOS Press. 2003. pp. 95-105.
Identification of differentially expressed genes by suppression subtractive hybridization (SSH). Desai, Hill, Trelogan, Diatchenko, Siebert. In: Functional Genomics: A Practical Approach. Editors: Hunt, Livesey. Oxford: Oxford University Press . 2000. pp. 81-112.
Update to: Differential screening of subtracted cDNA library: a rapid method to search for the genes preferentially expressed in two or multiple tissues. Diatchenko, Trelogan, Siebert. In: Expression Genetics: Accelerated and High-Throughput Methods . Editors: McClelland, Pardee. Natick: Biotechniques Books . 1999. pp. 141-144.
Differential screening of subtracted cDNA library: a rapid method to search for the genes preferentially expressed in two or multiple tissues. Jin, Cheng, Diatchenko, Siebert, Huang. In: Expression Genetics: Accelerated and High-Throughput Methods . Editors: McClelland, Pardee. Natick: Biotechniques Books . 1999. pp. 135-141.
Suppression subtractive hybridization: a versatile method for identifying differentially expressed genes. Diatchenko, Lukyanov, Lau, Siebert. In: Methods in Enzymology. Editors: Weissman . San Diego: Academic Press . 1999. pp. 349-380.
Suppression subtractive hybridization: a method for generating subtracted cDNA libraries starting from poly(A+) or total RNA. Diatchenko, Chenchik, Sebert. In: RT-PCR Methods for Gene Cloning and Analysis. Editors: Siebert, Larrick. Natick: Biotechniques Books . 1998. pp. 213-239.
Generation and use of high-quality cDNA from small amounts of total RNA by SMART PCR. Chenchik, Zhu, Diatchenko, Li, Hill, Siebert. In: RT-PCR Methods for Gene Cloning and Analysis. Editors: Siebert, Larrick. Natick: Biotechniques Books . 1998. pp. 305-321.
Books and Book Chapters
Peer-Reviewed Abstracts
Variable DNA methylation in genes involved immune response is a potential predictor of chronic widespread pain after motor vehicle collision Bortsov,Swift-Scanlan,Diatchenko,Swor,Peak,Jones,Rathlev,Lee,Domeier,Hendry,McLean. At: American Pain Society 33rd Annual Meeting Scientific Meeting. Tampa, Florida. 2014.
Genetic interaction between COMT and convergent molecular pathways influences COMT enzyme activity, musculoskeletal pain, and mood Smith,Reenila,Mannisto,Slade,Maixner,Diatchenko,Nackley. At: International Association for the Study of Pain 15th World Congress on Pain. Buenos Aires, Argentina. 2014.
Structural and functional interaction between 6TM MOR isoform and β2-adrenoceptors Samoshkin,Viet,Convertino,Maixner,Dokholyan,Schmidt,Diatchenko. At: 6th Study in Multidisciplinary Pain Research (SIMPAR) International Meeting. Rome, Italy. 2014.
Stress affects COMT haplotype dependent pain in a gender specific manner: a gene-sex-environment interaction Meloto,Bair,Slade,Maixner,Diatchenko. At: 6th Study in Multidisciplinary Pain research (SIMPAR). Rome, Italy. 2014.
Stress affects COMT haplotype dependent pain in a gender specific manner: a gene-sex-environment interaction Meloto,Segall,Tsao,Gauthier,Convertino,Reenila,Mannisto,Diatchenko. At: 18th Annual McGill Pain Day. Montreal, Canada. 2014.
R-bupranolol, an ADR-β1/2/3 antagonist, produces antinociception without sedation Piltonen,Martin,Gauthier,Maixner,Mogil,Diatchenko. At: 18th Annual McGill Pain Day. Montreal, Canada. 2014.
Genetic architecture of intermediate phenotypes associated with incident TMD in the OPPERA Prospective Study Smith,Mir,Bair,Slade,Dubner,Fillingim,Greenspan,Ohrbach,Knott,Weir,Maixner,Diatchenko. At: American Academy of Orofacial Pain 37th Scientific Meeting. Orlando, Florida. 2013.
Genetic association and meta-analysis implicates the ADRB2 adrenergic receptor gene in Irritable Bowel Syndrome (IBS) Smith,Diatchenko,Palsson,Kanazawa,vanTilburg,Maixner. At: American Pain Society Annual Scientific Meeting. New Orleands, LA. 2013.
Synergistic Analgesia Between Morphine and Beta-Adrenergic Antagonists Marcovitz,Wieskopf,Webb,Samoshkin,Dokholyan,Convertino,Stone,Mogil,Diatchenko. At: Annual Scientific Meeting, Society for Neuroscience. San Diego, CA. 2013.
How pharmacogenetics might help to predict postoperative pain and patient response to analgesia by morphine PCA: a case report DeGregori,DiMatteo,Allegri,Belfer,Diatchenko. At: 8th ISABS Conference on Forensic, Anthropologic and Medical Genetics. Split, Croatia. 2013.
COMT Haplotypes Predict Pain Intensity and Interference 6 Weeks after Motor Vehicle Collision Bortsov,Diatchenko,Swor,Peak,Jones,Rathlev,Lee,Domeier,Hendry,McLean. At: 32nd Annual Scientific Meeting, American Pain Society. New Orleans, LA. 2013.
Genetic association and meta-analysis implicates the ADRB2 adrenergic receptor gene in Irritable Bowel Syndrome (IBS) Smith,Diatchenko,Palsson,Kanazawa,vanTilburg,Maixner,Whitehead. At: 32nd Annual Scientific Meeting, American Pain Society. New Orleans, LA. 2013.
Characterization of a new COMT isoform associated with TMD Meloto,Segall,Gauthier,Tsao,Maixner,Diatchenko. At: 32nd Annual Scientific Meeting, American Pian Society. New Orleans, LA. 2013.
FKBP5 Polymorphisms Predict Peritraumatic Dissociation and Elevated PTSD Symptoms 6 Weeks After Motor Vehicle Collision Smith,Bortsov,Ulirsch,Swor,Peak,Jones,Rathlev,Lee,Domeier,Hendry,Diatchenko,Liberzon,McLean. At: Program number 243. Society of Biological Psychiatry 67th Annual Scientific Convention and Program EBook of Abstracts. Philadelphia, PA.
Sexually Dimorphic Influence of COMT on Distress in the Immediate Aftermath of Trauma Ballina,Bortsov,Diatchenko,Ulirsch,Swor,Peak,Jones,Rathlev,Lee,Domeier,Hendry,Liberzon,McLean. At: Program number 472. Society of Biological Psychiatry 67th Annual Scientific Convention and Program EBook of Abstracts. Philadelphia, PA.
Influence of COMT HPS Haplotype on Peritraumatic Distress Severity among Women Experiencing Motor Vehicle Collision: Report of an Age x Gene Interaction Platts-Mills,Ballina,Diatchenko,Bortsov,Swor,Jones,Rathlev,Lee,Domeier,Hendry,Liberzon. At: Program number 470. Society of Biological Psychiatry 67th Annual Scientific Convention and Program EBook of Abstracts. Philadelphia, PA.
Tryptophan Hydroxylase 2 Synonymous Polymorphism rs7305115 is Associated with Acute Psychological Response after Motor Vehicle Collision Bortsov,Soward,Diatchenko,Ulirsch,Swor,Peak,Jones,Rathlev,Lee,Domeier,Hendry,Liberzon,McLean. At: Program number 474. Society of Biological Psychiatry 67th Annual Scientific Convention and Program EBook of Abstracts. Philadelphia, PA.
FKBP5 Polymorphism Predicts Peritraumatic Dissociation after Motor Vehicle Collision Smith,Bortsov,Ulirsch,Swor,Peak,Jones,Rathlev,Lee,Domeier,Hendry,Diatchenko,Liberzon,McLean. At: Annual Meeting of the Society for Biological Psychiatry. Philadelphia, PA. 2011.
Tryptophan Hydroxylase 2 Synonymous Polymorphism rs7305115 is Associated with Acute Psychological Response after Motor Vehicle Collision Bortsov,Soward,Diatchenko,Ulirsch,Swor,Peak,Jones,Rathlev,Lee,Domeier,Hendry,Liberzon,McLean. At: Annual Meeting of the Society for Biological Psychiatry. Philadelphia, PA. 2011.
ADRA2A Variants Predict Peritraumatic Dissociation After Motor Vehicle Collision Ulirsch,Bortsov,Smith,Swor,Peak,Jones,Rathlev,Lee,Domeier,Hendry,Diatchenko,Liberzon,McLean. At: Annual Meeting of the Society for Biological Psychiatry. Philadelphia, PA. 2011.
Sexually Dimorphic Influence of COMT on Distress in the Immediate Aftermath of Trauma Ballina,Diatchenko,Ulirsch,Swor,Peak,Jones,Rathlev,Lee,Domieir,Hendry,Liberzon,McLean. At: Annual Meeting of the Society for Biological Psychiatry. Philadelphia, PA. 2011.
Influence of COMT HPS Haplotype on Peritraumatic Distress Severity among Women Experiencing Motor Vehicle Collision: Report of an Age x Gene Interaction Platts-Mills,Ballina,Diatchenko,Bortsov,Swor,Peak,Jones,Rathlev,Lee,Domieir,Hendry,Liberzon,McLean. At: Annual Meeting of the Society for Biological Psychiatry. Philadelphia, PA. 2011.
Serotonin-induced hyperalgesia via inhibition of catechol O-methyltransferase activity Tsao,Segall,Wieskopf,Mogil,Dokholyan,Diatchenko. At: 30th Annual Scientific Meeting, American Pain Society. Austin, TX. 2011.
Disruptive mRNA folding increases translational efficiency of catechol-O-methyltransferase variant Tsao,Diatchenko,Gauthier,Dokholyan,Shabalina. At: 30th Annual Scientific Meeting, American Pain Society. Austin, TX. 2011.
Pilot multicenter, genotype-based, randomized, double-blind, placebo-controlled trial of propranolol to reduce initial pain symptoms in patients with major burn injury Orrey,Shupp,Hoskins,Platts-Mills,Bortsov,Bangdiwala,Haith,Diatchenko,Holmes,Jordan,Cairns,McLean. At: 30th Annual Scientific Meeting, American Pain Society. Austin, TX. 2011.
Widespread palpation tenderness is associated with multiple pain conditions in females with temporomandibular disorders Chen,Slade,Maixner,Miller,Diatchenko. At: 30th Annual Scientific Meeting, American Pain Society. Austin, TX. 2011.
Contributions of inflammation mediators to localized TMD and widespread pain Slade,Diatchenko,Nackley. At: 89th General Session and Exhibition, International Association for Dental Research/American Association for Dental Research/Canadian Association for Dental Research. San Diego, CA. 2011.
Association of genetic polymorphisms with placebo treatment in patients with osteoarthritis knee pain Gracely,Smith,Slade,Diatchenko,Fillingim,Fijal,Frakes,McLean,Piezer,Heinloth,Maixner,Houston. At: 13th World Congress, International Association for the Study of Pain. Montréal, Québec, Canada. 2010.
Initial identification of genetic variants associated with painful osteoarthritis - results from a community based genome-wide association study Maixner,Smith,Bhangale,Weir,Hyde,Slade,John,McLean,Gracely,Ambrose,Tan,Jordan,Diatchenko. At: 13th World Congress, International Association for the Study of Pain. Montréal, Québec, Canada. 2010.
Multivariate factor analysis and genetic association provide evidence for clinical heterogeneity in fibromyalgia Smith,Fillingim,Maixner,Hyde,Slade,John,McLean,Gracely,Zaykin,Mazo,Yuryev,Tchivileva,Tan,Diatchenko. At: 13th World Congress, International Association for the Study of Pain. Montréal, Québec, Canada. 2010.
The role of age in TMD: a case-control study Miller,Slade,Diatchenko,Maixner. At: 13th World Congress, International Association for the Study of Pain. Montréal, Québec, Canada. 2010.
Cytokines associated with TMD case status and related intermediate phenotypes Nackley,Conrad,Slade,Smith,Gibson,Kasravi,Miller,Lim,Maixner,Diatchenko. At: 29th Annual Scientific Meeting, American Pain Society. Baltimore, MD. 2010.
A unified case-control association testing with unknown multilevel relationships Choi,Bhangale,Smith,Diatchenko,Maixner,Jordan,Hyde,John,Weir. At: 59th Annual Meeting, The American Society of Human Genetics. Honolulu, HI. 2009.
Activation of novel isoform of mu-opioid receptor (MOR1K) results in cellular excitation Gris,Cheng,Gauthier,Gibbson,Maixner,Diatchenko. At: Society for Neuroscience Annual Meeting. Chicago, IL. 2009.
Candidate genes associate with psychological risk factors for chronic pain in OPPERA, a multi-site population-based cohort study Smith,Fillingim,Slade,Greenspan,Ohrbach,Knott,Weir,Maixner,Diatchenko. At: Society for Neuroscience, 39th Annual Meeting. Chicago, IL. 2009.
Role of MOR-1K in Opioid Induced Hyperalgesia Conrad,Wentworth,Prusik,Gauthier,Sukumar,Maixner,Diatchenko,Nackley. At: Society for Neuroscience, 39th Annual Meeting. Chicago, IL. 2009.
Comt1 and pain perception in common inbred strains of mice Segall,Nackley,Diatchenko,Tarantino,Bailey,Steffy,Maynard,Wiltshire. At: Dental Research Day, School of Dentistry, University of North Carolina. Chapel Hill, North Carolina. 2009.
Candidate gene analysis of a persistent pain disorder Smith,Miller,Siddiqi,Gibson,Arunasalam,Kasravi,Neely,Bair,Slade,Maixner,Diatchenko. At: NIH Pain Symposium. Bethesda, MD. 2009.
Candidate genes associated with psychological risk factors for chronic pain – preliminary findings from the multisite OPPERA cohort study Smith,Fillingim,Greenspan,Ohrbach,Slade,Knott,Diatchenko,Maixner. At: 28th Annual Scientific Meeting, American Pain Society. San Diego, CA. 2009.
Propranolol in TMJD treatment Tchivileva,Lim,Kasravi,Gibson,Smith,Diatchenko,Maixner,McLean. At: American Pain Society, 28th Annual Meeting. San Diego, CA. 2009.
Cytokine Profiles Associated with TMD Case Status Nackley,Conrad,Gibson,Diatchenko,Maixner. At: 7th Annual Conference on Cytokines and Inflammation. San Diego, CA. 2009.
Proinflammatory Cytokine Profiles Associated with TMD Case Status and Related Intermediate Phenotypes Conrad,Arunasalam,Gibson,Bair,Smith,Slade,Maixner,Diatchenko,Nackley. At: American Pain Society, 27th Annual Meeting. Tampa, FL. 2008.
Functional characterization of the novel alternatively spliced form of mu-opioid receptor OPRM1 Gris,Cheng,Pierson,Gauthier,Shabalina,Spiridonov,Maixner,Diatchenko. At: American Pain Society, 27th Annual Meeting. Tampa, FL. 2008.
Catechol O-Methyltransferase (COMT) met/met genotype influences cortisol response and pain symptoms after minor motor vehicle collision (MVC) McLean,Diatchenko,Reed,Jones,Zaleski,Mistry,Swor,Sochor,Liberzon,Clauw,Maixner. At: American Pain Society, 27th Annual Meeting. Tampa, FL. 2008.
Effect of propranolol on TMD patients: preliminary findings Tchivileva,Lim,Diatchenko,McLean. At: American Pain Society, 27th Annual Meeting. Tampa, FL. 2008.
Candidate gene analysis reveals genetic pathways associated with a persistent pain disorder Smith,Siddiqi,Miller,Gibson,Arunasalam,Kasravi,Slade,Neely,Bair,Maixner,Diatchenko. At: American Society for Human Genetics, 58th Annual Meeting. Philadelphia, PA. 2008.
Candidate gene analysis of a persistent pain disorder Smith,Siddiqi,Miller,Gibson,Arunasalam,Kasravi,Slade,Neely,Bair,Maixner,Diatchenko. At: International Association for the Study of Pain, 12th World Congress on Pain. Glasgow, Scotland. 2008.
Functional characterization of the novel alternatively spliced form of mu-opioid receptor OPRM1 Gris,Cheng,Pierson,Gauthier,Shabalina,Spiridonov,Maixner,Diatchenko. At: International Association for the Study of Pain, 12th World Congress on Pain. Glasgow, Scotland. 2008.
Propranolol in TMD Treatment: Preliminary Findings Tchivileva,Lim,Kasravi,Smith,Diatchenko,Maixner,McLean. At: 5th Scientific Meeting of the TMJ Association. Bethesda, MD. 2008.
Detecting associations under models of complex traits prone to an effect reversal Zaykin,Shibata,Diatchenko. At: American Society for Human Genetics, 57th Annual Meeting. San Diego, CA. 2007.
Haplotype associations with quantitative traits in the presence of complex multilocus and heterogeneous effects Zaykin,Shibata,Diatchenko. At: American Society for Human Genetics, 57th Annual Meeting. San Diego, CA. 2007.
The NF-kB dependent regulation of catechol–O-methyltransferase expression Tchivileva,Sitcheran,Baldwin,Maixner,Diatchenko. At: American Pain Society, 26th Annual Meeting. Washington, DC. 2007.
ADRA1A polymorphisms associated with multiple psychological and nociceptive phenotypes predict vulnerability to an idiopathic pain conditions Smith,Slade,Belfer,Goldman,Max,Fillingim,Higgins,Sama,Maixner,Diatchenko. At: American Pain Society, 26th Annual Meeting. Washington, DC. 2007.
The role of NF-κB in modulating inflammatory pain and catechol-O-methyltransferase expression Nackley,Tchivileva,Conrad,Cooke,Maixner,Diatchenko. At: American Pain Society, 26th Annual Meeting. Washington, DC. 2007.
Effect of Minor SNPs on Enzymatic Activity Regulated by Common Human Haplotypes of the Catechol-O-methyltransferase Gene Lambert,Conrad,Shabalina,SA,Satterfield,Maixner,Diatchenko,Nackley. At: Society for Neuroscience 37th Annual Meeting. San Diego, CA. 2007.
Genetic and pharmacological modulation of the alpha-1a adrenergic receptor affects psychological and nociceptive risk factors for idiopathic pain Smith,Slade,Belfer,Goldman,Max,Fillingim,Maixner,Diatchenko. At: Society for Neuroscience 37th Annual Meeting. San Diego, CA. 2007.
Strategies for molecular identification and functional characterization of alternative splicing forms in human mu-opioid receptor OPRM1 Gris,Gauthier,Maixner,Shabalina,Spiridinov,Diatchenko. At: Society for Neuroscience 37th Annual Meeting. San Diego, CA. 2007.
The role of NF-κB in TNFα-regulated transcription of the human COMT in astrocytes Tchivileva,Sitcheran,Baldwin,Maixner,Diatchenko. At: American Society for Human Genetics, 56th Annual Meeting. New Orleans, LA. 2006.
NF-kB mediates TNFα inhibitory effect on human COMT gene expression Tchivileva,Sitcheran,Baldwin,Maixner,Diatchenko. At: American Society for Human Genetics, 55th Annual Meeting. Salt Lake City, UT. 2005.
Regulation of Adrenergic Receptor β2 by C/EBP and cAMP Pathways Higgins,Tchivileva,Downey,Maixner,Diatchenko. At: American Society for Human Genetics, 55th Annual Meeting. Salt Lake City, UT. 2005.
Common human catechol-O-methyltransferase haplotypes modulate RNA stability, protein expression, and enzymatic activity Nackley,Shabalina,Maixner,Diatchenko. At: American Society for Human Genetics, 55th Annual Meeting. Salt Lake City, UT. 2005.
Catechol-O-methyltransferase Inhibition Produces Enhanced Pain Sensitivity and Cytokine Production via a β-adrenergic Mechanism Nackley,Lambeth,Faison,Fecho,Diatchenko,Maixner. At: International Association for the Study of Pain, 11th World Congress on Pain. Sydney, Australia. 2005.
Catechol-O-methyltransferase modulates pain behavior and cytokine production via β2/3-adrenergic receptor mechanisms Nackley,Faison,Lambeth,Tan,Fecho,Flood,Diatchenko,Maixner. At: Society for Neuroscience 35th Annual Meeting. Washington, DC. 2005.
Catechol-O-methyltransferase haplotypes modulate pain sensitivity Nackley,Wu,Diatchenko,Maixner. At: Society for Neuroscience 34th Annual Meeting. San Diego, CA. 2004.
Reliable cDNA subtraction starting from small amounts of total RNA Diatchenko,Vijaychander,Chenchik,Siebert. At: Molecular Biology of the Cell, American Society for Cell Biology. . 1996.
Deciphering the role of melanocortin 1 receptor in pain through single nucleotide polymorphisms Lichtenwalter K,Lichtenwalter RN,Slade,Dubner,Fillingim,Greenspan,Ohrbach,Knott,Maixner,Diatchenko. At: Canadian Pain Society Scientific Meeting. Vancouver, Canada. 2016.
P2RX7 genetics at the crossroads of neuroimmune interactions Lichtenwalter K,Lichtenwalter RN,Ase,Niu,Komarova,Seguela,Slade,Dubner,Fillingim,Greenspan,Ohrbach,Knott,Maixner,Diatchenko. At: European Federation of IASP Chapters Scientific Meeting. Vienna, Austria. 2015.
The ne line between gain and loss: a genetic characterization of P2RX7 Lichtenwalter K,Lichtenwalter RN,Ase,Niu,Komarova,Seguela,Slade,Dubner,Fillingim,Greenspan,Ohrbach,Knott,Maixner,Diatchenko. At: Canadian Pain Society Scientific Meeting. Charlottetown, Canada. 2015.
The foul in fair and the fair in foul: gaining and losing pain with SNPs in P2RX7 Lichtenwalter K,Lichtenwalter RN,Ase,Niu,Komarova,Seguela,Slade,Dubner,Fillingim,Greenspan,Ohrbach,Knott,Maixner,Diatchenko. At: McGill Pain Day. Montreal, Canada. 2015.
The role of P2RX7 genetic variants in pain processing examined through an annotated catalogue of non-synonymous SNPs Lichtenwalter K,Lichtenwalter RN,Ase,Niu,Komarova,Seguela,Slade,Dubner,Fillingim,Greenspan,Ohrbach,Knott,Maixner,Diatchenko. At: International Association for the Study of Pain Conference. Buenos Aires, Argentina. 2014.
Peer-Reviewed Abstracts
News

News


Katerina Zorina-Lichtenwalter selected for oral presentationMay 24, 2016

Katerina Zorina-Lichtenwalter is selected to give an oral presentation on "Deciphering the role of melanocortin 1 receptor in pain through single nucleotide polymorphisms" during the Hot Topics Sessions at the annual Canadian Pain Society meeting in Vancouver, Canada.

Carol Meloto receives Kresimir Krnjevic Research AwardMay 18, 2016

Dr. Carol Meloto receives the 14th Kresimir Krnjevic Research Award for 2016.

Carol Meloto receives Catherine Bushnell Fellowship in Chronic Pain ResearchMay 17, 2016

Dr. Carol Meloto receives the Catherine Bushnell Fellowship in Chronic Pain Research for 2016.

Katerina Zorina-Lichtenwalter receives QPRN travel grantApril 2016

Katerina Zorina-Lichtenwalter is awarded a travel grant of $1000 from the Quebec Pain Research Network (QPRN) to attend the Canadian Pain Society Meeting from May 24-27, 2016.

Carol Meloto wins best young researcher award2015

Dr. Carol Meloto wins Best Young Researcher at the 7th Study in Multidisciplinary Pain Research (SIMPAR) in Rome, Italy.

Publication of Pain Genetics: Basic to Translational ScienceJanuary 2014

Pain Genetics: Basic to Translational Science is a timely synthesis of the key areas of research informing our understanding of the genetic basis of pain. The book opens with foundational information on basic genetic mechanisms underlying pain perception and progresses recently discovered complex concepts facing the field. The coverage is wide-ranging and will serves as an excellent entry point into understating the genetics of pain as well as providing a single resource for established researchers looking for a better understanding of the diverse strands of research going on in the area. With contributors painstakingly selected to provide a broad range of perspectives and research, Pain Genetics will be a valuable resource for geneticists, neuroscientists, and biomedical professionals alike.

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Human pain genetics focus of new excellence research chairSeptember 30, 2013

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$10-million federal grant brings prominent pain researcher to McGillSeptember 26, 2013

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World-leading expert on pain genetics joining McGillSeptember 25, 2013

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Studies

Clinical Studies


Fibromyalgia Study

Opportunities


The Human Pain Genetics lab forms part of the Alan Edwards Centre for Research on Pain at McGill University, which includes researchers from the Faculties of Medicine, Dentistry and Science all centered on the study of pain. Being part of this prestigious center not only facilitates an excellent environment for collaborations but also creates unique learning opportunities and offers exposure to a variety of approaches in pain research.

We encourage applicants with backgrounds from a variety of fields including: epidemiology, bioinformatics, statistics, statistical genetics, medicine, and nursing.

If you wish to inquire about possible training opportunities with our group please follow instructions below. Any questions should be addressed to Sarah Martinez.

Undergraduate Studies

If you are an undergraduate looking to do honors research projects, please send the following to Sarah Martinez:

  • a cover letter
  • a current CV

Graduate Studies

If you are interested in applying for a Master's or PhD program with our group, please send the following to Luda Diatchenko:

  • a cover letter
  • a current CV
  • the e-mail address of one (1) reference

Postdoctoral Studies

If you are interested in becoming a postdoctoral researcher in our lab, please send the following to Luda Diatchenko:

  • a cover letter
  • a current CV
  • the e-mail addresses of three (3) references
  • a one-paragraph statement of interest in the lab
  • a one-paragraph statement of scientific accomplishments

Clinical Fellows

Medical residents and other trainees with healthcare backgrounds who wish to receive clinical research experience, please send the following to Luda Diatchenko:

  • a current CV
  • a one-paragraph statement of interest in the lab

Life in Montreal

Notre Dame of Montreal
Allez Up Climbing Gym
Montreal at Night
Another Montreal Picture

The great city of Montreal has been home to Dr. Diatchenko's Human Pain Genetics lab since 2013. Montreal is a vibrant place that contrasts the cosmopolitan feeling of a big North American city with the local charm of the Québécois culture. We enjoy exploring the city during all seasons, and members of the lab often get together to enjoy urban markets, cultural events, and outdoor activities, such as hiking, biking, and climbing. Montreal is one of the top host cities for international events in North America, with festivals and activities happening all year such as the Montreal Jazz Festival, the Montreal Grand Prix and the International Festival of Circus Arts. The city also ranks highest in Canada for the the number of restaurants per capita, and we plan to try them all!

Resources


Human Pain Genetics Database

The Human Pain Genes Database (HPGDB) is a comprehensive compilation of single nucleotide polymorphisms (SNPs) that have been reported to be associated with pain. Interpersonal variability in chronic pain etiology, acute pain sensitivity, and analgesic response has been suggestive of differences in genetic predisposition, a theory supported by the numerous genetic association studies published during the last twenty years. As common, generally low-effect markers of genetic variability, SNPs are instrumental in sampling this predisposition and in identification of risk-modifying genes. With an ever-growing number of studies, the HPGDB is a regularly-updated tabular summary of SNP-phenotype associations, populated via manually-curated literature review. It is intended to serve as a reference and to generate new hypotheses that may lead to a better understanding of underlying molecular mechanisms and consequently the development of personalized treatment.

Results are presented in the following six-column format: genetic locus (or loci where applicable), SNP rsID, allele or haplotype reported in association analysis, direction of effect, associated phenotype, and citation. Additional information is displayed upon hovering over each field, and fields provide links to the corresponding reference database in NCBI and PubMed. The data is downloadable in a variety of formats.

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DRG-eQTLs

Single nucleotide polymorphisms (SNPs) associated with the expression level of a gene or an exon are called expression quantitative trait loci (eQTLs). We have mapped eQTLs in human dorsal root ganglions (DRG), a tissue highly relevant for pain research. This resource provides processed eQTL data (SNP, gene, beta, p-value) and input files for matrix_eQTL (genotype, mRNA levels, covariates).

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Contact Us

Contact Us

Human Pain Genetics Lab
Alan Edwards Centre for Research on Pain
Genome Building, Room 2201, 740 Dr. Penfield Avenue
Montreal, Quebec, Canada H3A 0G1
T: 514-398-2878 | F: 514-398-8900